Journal: Scientific Reports

Article Title: Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5

doi: 10.1038/s41598-018-23194-9

Figure Lengend Snippet: Phagocytosis assay by THP-1 cells. ( a ) (i) Western blotting of bound protein(s) on latex beads after solubilization. Lane 1: SE36-beads, lane 2: SE36-beads with VTN, lane 3: latex-beads alone, lane 4: beads with VTN. SE36 and VTN bound to the beads were detected by anti-SE36 mouse serum (diluted 1:1000) and anti-VTN pAb (diluted 1:2000), respectively. Arrows indicate the target proteins. (ii) Representative flow cytometry (FCM) histogram of the antibody-independent engulfment of SE36-beads with or without VTN by THP-1 cells. (iii) Giemsa staining of engulfed SE36-beads (left panel) and non-treated beads (right panel) by THP-1 cells. Arrows indicate the engulfed SE36-beads. Scale bar, 10 μm. ( b ) FCM histogram of the engulfment by THP-1 cells of SE36-beads with or without VTN and/or purified IgG from NHS or HTS. ( c ) FCM histogram of the engulfment by THP-1 cells of SE36-beads treated with normal serum or VTN-depleted serum (VTN-depl). ( d ) FCM histogram of the engulfment by THP-1 cells of SE36-beads with or without VTN-depl, with or without purified VTN or with normal serum.

Article Snippet: Purified VTN was purchased from Promega (Madison, WI, USA), and VTN recombinants, Recombinant Human Vitronectin (62–398 aa) Protein (Cat #: HRP-0323) and Recombinant Human Vitronectin Protein, CF (Cat #: 2308-VN), were obtained from LD Biopharma (San Diego, CA, USA) and R&D systems (Boston, MA, USA), respectively.

Techniques: Phagocytosis Assay, Western Blot, Flow Cytometry, Staining, Purification

Journal: Scientific Reports

Article Title: Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5

doi: 10.1038/s41598-018-23194-9

Figure Lengend Snippet: Binding assay of vitronectin (VTN) to SE36. ( a ) Reactivity of the purified VTN and HSA against SE36. SE36 was adsorbed to microtiter plate and VTN or human serum albumin (HSA) was added at the indicated concentrations. ( b ) Western blotting of elutes from SE36-immobilized and control columns. The anti-VTN pAb detected two forms (V75 and V65; upper band and lower band, respectively) of VTN. Each elute was run in an SDS-PAGE gel and probed with anti-VTN pAb (diluted 1:2000). ( c ) Reactivity of VTN in naïve human serum (NHS) and Ugandan high anti-SE36 IgG-titer serum (HTS) against SE36. SE36 was adsorbed to microtiter plate and NHS or HTS was added at the indicated dilutions (closed symbols). Open symbols show reactions without anti-VTN Ab as negative control. ( d ) Reactivity of commercially available VTN recombinants (see also Fig. 1e) against SE36. SE36 was adsorbed to microtiter plate and VTN recombinants were added at the indicated concentrations. ( e ) Schematic representation of VTN. “SmB”, “Hp”, and “Hb” indicate Somatomedin-B motif, Hemopexin domain, and Heparin-binding region, respectively. The number 398 denotes the endogenous cleavage site. The designations “20–478” and “62–398” indicate commercially available VTN recombinants. VTN-1 to -4, as well as VTN-2-1 to -2-3 denote truncated recombinant forms. Results in ( a , c , and d ) are expressed as means ± SD from three independent experiments. In ( c ), statistical analysis between NHS and HTS was performed using a Mann-Whitney U test. No significant differences were found.

Article Snippet: Purified VTN was purchased from Promega (Madison, WI, USA), and VTN recombinants, Recombinant Human Vitronectin (62–398 aa) Protein (Cat #: HRP-0323) and Recombinant Human Vitronectin Protein, CF (Cat #: 2308-VN), were obtained from LD Biopharma (San Diego, CA, USA) and R&D systems (Boston, MA, USA), respectively.

Techniques: Binding Assay, Purification, Western Blot, Control, SDS Page, Negative Control, Recombinant, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5

doi: 10.1038/s41598-018-23194-9

Figure Lengend Snippet: Mapping of binding site of VTN in SE36. ( a ) Schematic representation of SE36. The “8” and “S” indicate octamer repeat and serine rich region, respectively. “RGD” indicates RGD motif. The number 178 denotes the position of polyserine sequence present in PfSERA5 but deleted in SE36 . SE36-1 to -4 denote truncated recombinant forms. P1 to P15 are synthetic peptides . ( b ) Reactivity of VTN in NHS and HTS against each truncated SE36 recombinant. Each recombinant was adsorbed to microtiter plate and NHS and HTS were added at 1:2000 dilution. ( c ) Reactivity of the purified VTN against each truncated SE36 recombinant. Each recombinant was adsorbed to microtiter plate and the purified VTN was added at 2 μg/mL concentration. ( d ) Reactivity of VTN in NHS and HTS against each synthetic peptide. Each synthetic peptide was adsorbed to microtiter plate (0.3 μM) and NHS and HTS were added at 1:2000 dilution. ( e ) Reactivity of the purified VTN against each synthetic peptide. Each synthetic peptide was adsorbed to microtiter plate and the purified VTN was added at 2 μg/mL concentration. Results in ( b – e ) are expressed as means ± standard deviation (SD) from three independent experiments. In ( b and d ), statistical analyses between NHS and HTS were performed using a Mann-Whitney U test. No significant differences were found.

Article Snippet: Purified VTN was purchased from Promega (Madison, WI, USA), and VTN recombinants, Recombinant Human Vitronectin (62–398 aa) Protein (Cat #: HRP-0323) and Recombinant Human Vitronectin Protein, CF (Cat #: 2308-VN), were obtained from LD Biopharma (San Diego, CA, USA) and R&D systems (Boston, MA, USA), respectively.

Techniques: Binding Assay, Sequencing, Recombinant, Purification, Concentration Assay, Standard Deviation, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5

doi: 10.1038/s41598-018-23194-9

Figure Lengend Snippet: Characterization of VTN binding to SE36. ( a ) Biophysical interaction analysis of the binding of VTN to SE36 using surface plasmon resonance (SPR). The raw data (color lines) were fitted to 1:1 binding and bivalent analyte model (black lines) using the Biacore T200 Evaluation software. The fit parameters from these models are summarized in ( b ). These experiments were performed in triplicate, and representative data are shown. ( b ) Kinetic and equilibrium constants for the binding of VTN to SE36. ( c ) Schematic representation of two schemes in bivalent binding model. (i) VTN contains two binding sites. (ii) VTN contains a single binding site and forms a dimer. ( d ) (i) Purity of VTN-1 to -4 recombinants confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue (CBB) staining. Two micrograms of each recombinant was run in a gel. Arrows indicate target proteins. Since estimated molecular weights were 18, 21, 14, and 12 kDa in VTN-1 to -4, respectively, it was presumed that these recombinants were glycosylated in Pichia pastoris . (ii) Reactivity of SE36 against each truncated VTN recombinant. Each recombinant was adsorbed to microtiter plate (1 μg/mL) and SE36 was added at the indicated concentrations. ( e ) (i) Purity of VTN-2-1 to -2-3 recombinants confirmed by SDS-PAGE and CBB staining. Two micrograms of each of recombinant was run in a gel. Arrows indicate target proteins. Since estimated molecular weights were 8 kDa in VTN-2-1 to -2-3, it was, likewise, presumed that these recombinants were glycosylated in P. pastoris . All recombinant proteins of VTN ( d and e ) were detected by anti-His tag antibody in western blotting and was purified by His GraviTrap. (ii) Reactivity of SE36 against each truncated VTN recombinant. Each recombinant was adsorbed to microtiter plate (1 μg/mL) and SE36 was added at the indicated concentrations.

Article Snippet: Purified VTN was purchased from Promega (Madison, WI, USA), and VTN recombinants, Recombinant Human Vitronectin (62–398 aa) Protein (Cat #: HRP-0323) and Recombinant Human Vitronectin Protein, CF (Cat #: 2308-VN), were obtained from LD Biopharma (San Diego, CA, USA) and R&D systems (Boston, MA, USA), respectively.

Techniques: Binding Assay, SPR Assay, Software, Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Recombinant, Western Blot, Purification